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KMID : 0385020030190030161
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Korean Journal of Laboratory Animal Science
2003 Volume.19 No. 3 p.161 ~ p.167
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Histological analysis on the gross brain morphology of the tremor mutant mice generated by ENU mutagenesis
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Park Young-Sook
Cho Kyu-Hyuk
Cho Jae-Woo
Song Chang-Woo
Shin Tae-Kyun
Park Chan-Kyu
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Abstract
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The newly identified ENU-induced mutant mouse showing tremor phenotype was analyzed by histological and immunohistological analyses to evaluate possible pathological abnormalities in the brain. There were no gross morphological differences in the regions including neocortex, olfactory bulb, hippocampus, thalamus, cerebellum and midbrain between wildtype and tremor mutants from the comparison made by hematoxylin/eosin staining and light microscopic analysis. For the cerebellum, immunostaining using the calbindin-D28 antibody was performed to evaluate Purkinje cell abnormality. There was no cerebellar developmental abnormality in the tremor mutant mice. There was also no difference in the myelination pattern studied by luxol fast blue staining between controls and mutant mice, which is one of the major causes associated with tremor. There was no picnotic cells identifiable by hematoxylin/eosin staining. Therefore, we concluded that there exist no obvious developmental and morphological defects in the tremor mutant mice examined in this study. To further understand the underlying mechanisms of this newly identified tremor mutant mouse, the genetic analysis of the mutation is necessary.
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KEYWORD
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cerebellum, ENU, mutagenesis, tremor
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